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The extract obtained cells can be used either directly or be purified further. After reaching the stationary phase cells were harvested by centrifugation and separated from the medium.

The coenzyme regeneration is made in this respect by the simultaneous oxidation of secondary alcohols, being used in most cases the 2-propanol miscible with water, economic.

The reaction mixtures for ee determination were extracted after 24 hours h for example with chloroform and by gas chromatography GC the enantiomeric excess was determined.

Sep de ; 62 4: The cells used in this respect may be native, permeabilized or lysed way. Ketoreductase polypeptides for the production of 3-arylhydroxypropanamine from a 3-arylketopropanamine. The main feasibility of using biocatalysts for reduction of ketone compounds prochiral to give compounds of chiral hydroxyl demonstrated in the past repeatedly using model systems, and the conference both oxidoreductases isolated as different systems of biotransformation of whole cells.

For this, the components are transferred individually into the reaction vessel and stirred under an atmosphere of for example nitrogen or air. Algunas enzimas que contienen al cofactor de molibdeno son la xantina oxidasaDMSO reductasasulfito oxidasa y nitrato reductasa. For this, the aqueous phase is separated, the organic phase is filtered.

The reaction was continued for 1 min at nm. Therefore, even homologous enzymes proportionally similar may occur with respect to a particular substrate behavior completely different reaction. Amplification and subsequent reamplification of specific cDNA results in a fragment that binds to the analysis in a pCR2.


Activity testing is composed as follows: In another preferred embodiment of the methods according to the invention the enzymatic reaction in the presence of a water-immiscible or water-miscible organic solvent only limited performed. Novel enzymes, a method of producing the enzyme, dna encoding the enzyme, a transformant containing the dna, method for producing such optically active alcohols by the enzyme.

Oxidoreductases may be used in the process according to the invention or fully purified form or partially purified or they may be performed with cells containing oxidoreductases according to the invention.

La prueba de actividad se compone tal como sigue: Production by rDNA technology and application in cheese manufacture”. In this respect it is recorded particularly in the pharmaceutical industry a growing need for chiral compounds and thus chiral synthesis technologies, since in the future and only be used as drugs racemic compounds.

American Association of Cereal Chemists,pp. After evaporating the solvent optionally the filtered organic phase. Asimismo, tienen un papel relevante en la manufactura de materias primas de la industria alimentaria. Reaction mixture for the determination of ee value: Generally, these enzymes dependent cofactors.

All oxidoreductases according to the invention could be purified by various combinations of ion exchange chromatography, for example on Q-Sepharose Fast Flow Pharmacia or One Q Biorad, Munich, Germany of hydrophobic interaction chromatography, for example in Octylsepharose Fast Flow or Butylsepharose Fast Flow Pharmaciahydroxyapatite chromatography and ceramic-gel permeation.

De Wikipedia, la enciclopedia libre. Los restos bifenililo, restos naftilo y en particular restos fenilo son restos arilo preferentes. The reaction mixture, in case of using water-insoluble solvents or co-substrates, is composed of an aqueous phase and an organic phase.

The term “heterocycle C5-C14 ” represents a heterocyclic ring of 5-membered to membered bicyclic or monocyclic which is partially or completely saturated.

Molibdopterina – Wikipedia, la enciclopedia libre

Enzyme release was performed by wet grinding by means of glass beads, but may also be achieved by other methods disintegration. By the term aryl residues of aromatic hydrocarbon it is meant having 6 to 14 carbon atoms in the ring.


By the term ” C3-C7 ” cyclic hydrocarbon residues such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl means. In Table 5 the results are grouped for oxidoreductases according to the invention.

Metabolismo de la xilosa

Accordingly absent information sequence on the 5 ‘end of the gene is obtained. Tools for Biotechnological Processes”, Biomolecules, 4, pp. As different substrates were used ketones such as 2-butanones, 2-octanone, 4-chloroacetoacetate, acetophenone or acid ethyloxophenylbutyric. The recombinant protein expression was induced by adding isopropylthiogalactoside IPTG at a concentration of 0.

Biotechnology Advances, 17, pp. Examples Ry oxidoreductases and dehydrogenase S-specific suitable high enantioselectivity: Adicionalmente el anillo pirano puede presentar dos tiolatoslos cuales sirven como ligandos en las enzimas con molibdeno y tungsteno. Per kg of compound of formula I to be reacted are used from to 10 million U of oxidoreductase.

Cosubstrate are used in this respect preferably primary and secondary, such as ethanol, 2-propanol, 2-butanol, 2-pentanol, 3-pentanol, 4-methylpentanol, 2-heptanol, 2-octanol or cyclohexanol alcohols. Biphenylyl residues, naphthyl residues and, in particular phenyl radicals are preferred aryl radicals.

Enzymatic reduction itself takes place in mild conditions, so that the alcohols do not react subsequently generated. Tabla 1 Table 1. The aqueous phase can be extracted again optionally as the organic phase can be further processed.

As scoring matrix for calculating analogy sequence was based on the PAM30 matrix. Asymmetric reduction of ketones using recombinant E.